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Chinese Scientists Develop A New Fluorescence Method to Assist Environmental Monitoring TEXT SIZE: A A A
Date:2018.06.01 Author:GAI Yanbo Clicks:

A Chinese research team developed a new fluorescence method to rapidly measure the phytoplankton photosynthetic rate in situ.

This highly efficient method could meet the rapid observation demand in ecological and environmental monitoring.

Phytoplankton are the self-feeding components of the plankton community and take a key part of oceans, seas and freshwater basin ecosystems.

And its photosynthesis is the basis of the material circulation and ecosystem energy flow, and affects the global biogeochemical cycle and climate change fundamentally.

The rapid and accurate measurement of phytoplankton photosynthetic rate is of great significance for water ecological environment monitoring, marine resource assessment and global climate change prediction.

In the traditional gas exchange methods, the photosynthetic rate is calculated by measuring the photosynthetic oxygen evolution rate or the photosynthetic carbon fixation rate.

These methods are always too time consuming and inefficient to meet the rapid observation demand in ecological and environmental monitoring.

Dr. YIN Gaofang, the first author of the paper as well as a scientist with Anhui Institute of Optics and Fine Mechanics (AIOFM), Hefei Institutes of Physical Science put forward a photosynthetic rate measurement method based on chlorophyll fluorescence induced by dynamic light.

Dynamic light was used to regulate the photosynthetic energy transfer process, and the induced fluorescence was employed to measure the photosynthetic energy flow rate, which indicates the physiological state of phytoplankton living cells.

The newly-proposed method could get the problems in gas exchange with the advantages of rapid measurement, non-pretreatment, non-pollution and non-invasion

It is an effective method to measure the phytoplankton photosynthetic rate in situ, thus possesses a broad prospect for applications in water ecological environment monitoring, marine resource assessment and global climate change prediction.

"This method is also applicable to higher plants and has wide application prospects in plant physiology and pathology research", said Prof. ZHAO Nanjing, the corresponding author of the paper, who also leads the study team.

The study is sponsored by National Key Research and Development Program of China (2016YFC1400600), the Open Fund of Qingdao National Laboratory for Marine Science and Technology (QNLM2016ORP0312), National "863" Program of China (2014AA06A509), Natural National Science Foundation of China (31400317) and Natural Science Foundation of Anhui Province (1708085QD87).

Link to the paper: Phytoplankton photosynthetic rate measurement using tunable pulsed light induced fluorescence kinetics

Figure1. Diagram of tunable pulsed light induced fluorescence kinetics measurement system (Image by YIN Gaofang)

Figure2. Measurement process of tunable pulsed light induced fluorescence kinetics (Image by YIN Gaofang)

 
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